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merck millipore,默克密理博,S8001,CpGenome™ Human Methylated & Non-Methylated DNA Standard Set
产品名称：CpGenome™ Human Methylated & Non-Methylated DNA Standard Set
产品型号：S8001
Purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) & DNMT3b (-/-) & have <5% methylated DNA.
merck millipore,默克密理博,S8001,CpGenome™ Human Methylated & Non-Methylated DNA Standard Set
- 产品介绍
描述 产品目录编号 S8001 品牌系列 Upstate
商名 - Upstate
- CpGenome
描述 CpGenome™ Human Methylated & Non-Methylated DNA Standard Set 概述 CpGenome™ Human Methylated & Non-methylated DNA Standards are purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. The CpGenome™ Human Methylated DNA Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase. These standards are intended for use as positive and negative controls in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome™ Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ® amplification kits.
Materials Provided:
Two vials, each containing 5 µg (20 µl) of CpGenome™ Human Non-Methylated DNA Standard or 5 µg (20 µl) of CpGenome™ Human Methylated DNA Standard, at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard, and only the U primer set amplified the unmethylated Standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.产品信息 演示 Aqueous buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 应用 应用 Purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) & DNMT3b (-/-) & have <5% methylated DNA. 应用说明 For MSP primer design, please use the MethPrime software package. 生物信息 品种反应性 Human 修改 - Methylation
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended Storage: Stable for 6 months at -20°C from date of receipt. 包装信息 数量 5 µg per vial
上一件merck millipore产品：merck millipore,默克密理博,648311,Tris Base, ULTROL Grade - CAS 77-86-1 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,341298,FASTsilver™ Gel Staining Kit
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