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merck millipore,默克密理博,MAB397,Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4
产品名称：Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4
产品型号：MAB397
Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB.
merck millipore,默克密理博,MAB397,Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, M, R, Mk WB, ELISA, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB397 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 Alternate Names - GluR2
背景信息 Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses. 产品信息 格式 Purified 控制 - Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
演示 Purified mouse immunoglobin IgG2a liquid in buffer containing PBS, no preservative. 应用 应用 Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry:
on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).
ELISA/RIA:
A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).
Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).
Immunoprecipitation: 2-4 µg/mL (Osten, 1998).
Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.
Optimal working dilutions and protocols must be determined by end user.生物信息 免疫原品种 Recombinant fusion protein TrpE-GluR2 (putative N-terminal portion, amino acids 175-430). 表位 Extracellular 克隆 6C4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2. Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996). 同种型 IgG2a 品种反应性 CanineMouseRatMonkey Species Reactivity Note Expected to react with rat, mouse and canine based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_058957
Entrez基因汇总 Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. 基因符号 - GluR-K2
- Gria2
- GLUR2
- GLURB
- GluR-2
- GluR-B
纯化方法 Purified immunoglobulin from culture supernatant UniProt编号 - P19491
UniProt汇总 FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
SIZE: 883 amino acids; 98,688 Da
SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.
TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.
SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. ...产品使用声明 质量保证 Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,AB3572P,Anti-Organic Anion Transporting Polypeptide 2 Antibody, CT, intracellular
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