merck millipore,默克密理愽
  • merck millipore,默克密理博,05-369,Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

  • merck millipore,默克密理博,05-369,Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

    产品名称：Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6
    产品型号：05-369
    Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.

    merck millipore,默克密理博,05-369,Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

  • 产品介绍
  • merck millipore,默克密理博,05-369,Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Sh, H, R, Rb, M, Mk, Xn, Po, CaWB, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-369
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6
    Alternate Names
    • sodium/potassium-transporting ATPase alpha-3 chain
    • ATPase, Na+/K+ transporting, alpha 3 polypeptide
    • Na(+)/K(+) ATPase alpha(III) subunit
    • Na(+)/K(+) ATPase alpha-3 subunit
    • Na+/K+ -ATPase alpha 3 subunit
    • Na+/K+ ATPase 3
    • Sodium pump subunit alpha-3
    • dystonia 12
    • sodium pump 3
    • sodium-potassium-ATPase, alpha 3 polypeptide
    • sodium pump 3
    • sodium-potassium-ATPase
    背景信息 Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
    In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
    产品信息
    格式 Purified
    控制
    • Alpha 1 mRNA is present in all cell types examined.
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at
    -20ºC.
    Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.
    应用
    应用 Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunoprecipitation:
    2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.

    Immunohistochemistry:
    1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.

    1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
    2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
    3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.
    生物信息
    免疫原品种 Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
    克隆 C464.6
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.
    同种型 IgG1κ
    品种反应性 SheepHumanRatRabbitMouseMonkeyXenopusPigCanine
    Species Reactivity Note Rabbit, Human, Monkey, Mouse, Rat, Dog, Lamb, Pig, Xenopus.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_001001586
    Entrez基因汇总 The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
    基因符号
    • DYT12
    • ATP1A3
    • MGC13276
    • RDP
    • EC 3.6.3.9
    纯化方法 Protein G chromatography
    UniProt编号
    • P05023
    UniProt汇总 FUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
    SIZE: 1013 amino acids; 111749 Da
    SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
    SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
    DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
    SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
    产品使用声明
    质量保证 Routinely evaluated in western blot on rat brain microsomal preparations.

    Western Blot Analysis:
    0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.

    Handling Recommendations:
    Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,05-369,Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

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