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merck millipore,默克密理博,MABD168,Anti-WNT1 Antibody, clone 10C8
产品名称：Anti-WNT1 Antibody, clone 10C8
产品型号：MABD168
Detect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
merck millipore,默克密理博,MABD168,Anti-WNT1 Antibody, clone 10C8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H FC, WB, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABD168 描述 Anti-WNT1 Antibody, clone 10C8 Alternate Names - Proto-oncogene Int-1 homolog
- Proto-oncogene Wnt-1
背景信息 Wnt-1, also known as Proto-oncogene Wnt-1 or Proto-oncogene Int-1 homolog, was first identified as a proto-oncogene activated by viral insertion in mouse mammary tumors. The Wnt-1 gene encodes a member of a large family of secreted proteins that are cysteine-rich, glycosylated, and poorly soluble. Wnt-1 serves as a ligand for the RYK coreceptor, resulting in Wnt signaling. The Wnt signalling pathway mediates the levels of cytosolic beta-catenin, a multi-functional protein that associates with membrane-bound E-cadherin as well as several DNA-binding proteins, such as members of the TCF/LEF family. Additionally, it serves a ligand for members of the frizzled family of seven transmembrane receptors and plays a role in development of the central nervous system. 产品信息 格式 Ascites 控制 - NIH/3T3, 3T3L1, and HeLa cell lysates
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Detect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
应用说明 Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in HeLa and 3T3-L1 cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in human adrenal tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected WNT1 in HeLa cells.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Purified recombinant fragment of human WNT1 expressed in E. Coli. 克隆 10C8 宿主 Mouse 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody 基因符号 - WNT1
- INT1
纯化方法 Unpurified UniProt编号 - P04628
产品使用声明 质量保证 Evaluated by Western Blotting in NIH/3T3, 3T3L1, and HeLa cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody detected WNT1 in NIH/3T3, 3T3L1, and HeLa cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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