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merck millipore,默克密理博,05-1501,Anti-MAD2A Antibody, clone 17D10
产品名称：Anti-MAD2A Antibody, clone 17D10
产品型号：05-1501
Use Anti-MAD2A Antibody, clone 17D10 (Mouse Monoclonal Antibody) validated in WB, IP to detect MAD2A also known as MAD2 mitotic arrest deficient-like protein 1 (yeast).
merck millipore,默克密理博,05-1501,Anti-MAD2A Antibody, clone 17D10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1501 描述 Anti-MAD2A Antibody, clone 17D10 Alternate Names - mitotic arrest deficient, yeast, homolog-like 1
- MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
- MAD2 mitotic arrest deficient-like 1 (yeast)
- MAD2-like 1
- MAD2-like protein 1
背景信息 MAD2A is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. MAD2A then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately cell death or disease. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-MAD2A Antibody, clone 17D10 (Mouse Monoclonal Antibody) validated in WB, IP to detect MAD2A also known as MAD2 mitotic arrest deficient-like protein 1 (yeast). 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated MAD2A from 100 μg of HEK293 cell lysate. 生物信息 免疫原品种 Recombinant protein corresponding to full length human MAD2A. 克隆 17D10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes MAD2A. 同种型 IgG1κ 品种反应性 MouseHumanRat Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - Q13257
基因符号 - MAD2
- HSMAD2
- HsMAD2
纯化方法 Protein G purfied UniProt编号 - Q13257
UniProt汇总 FUNCTION: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
SUBUNIT STRUCTURE: Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR.
SUBCELLULAR LOCATION: Nucleus. Kinetochore. Cytoplasm. Note: Recruited by MAD1L1 to unattached kinetochores Probable. Recruited to the nuclear pore complex by TPR during interphase.
DOMAIN: The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
PTM: Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
SEQUENCE SIMILARITIES: Belongs to the MAD2 family.
Contains 1 HORMA domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MAD2A in 15 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
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