merck millipore,默克密理愽
  • merck millipore,默克密理博,ABE489,Anti-dimethyl G9a Antibody (Lys185)

  • merck millipore,默克密理博,ABE489,Anti-dimethyl G9a Antibody (Lys185)

    产品名称：Anti-dimethyl G9a Antibody (Lys185)
    产品型号：ABE489
    Detect G9a using this rabbit polyclonal antibody, Anti-dimethyl G9a Antibody (Lys185) validated for use in western blotting, Peptide Inhibition Assay & IP.

    merck millipore,默克密理博,ABE489,Anti-dimethyl G9a Antibody (Lys185)

  • 产品介绍
  • merck millipore,默克密理博,ABE489,Anti-dimethyl G9a Antibody (Lys185)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 ABE489
    描述 Anti-dimethyl G9a Antibody (Lys185)
    Alternate Names
    • Protein G9a
    • Histone-lysine N-methyltransferase EHMT2
    • Euchromatic histone-lysine N-methyltransferase 2
    • HLA-B-associated transcript 8
    • Histone H3-K9 methyltransferase 3
    • H3-K9-HMTase 3
    • Lysine N-methyltransferase 1C
    背景信息 G9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. The presence of the ankyrin repeats also suggests that G9a is involved in intracellular protein-protein interaction. Like Suv39h1, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10- to 20-fold higher activity. G9a is localized to the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39h1 family proteins were localized. This protein is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a also methylates histone H1.
    产品信息
    格式 Affinity Purified
    控制
    • HEK293 nuclear extract
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Detect G9a using this rabbit polyclonal antibody, Anti-dimethyl G9a Antibody (Lys185) validated for use in western blotting, Peptide Inhibition Assay & IP.
    主要应用
    • Western Blotting
    应用说明 Western Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected dimethyl G9a (Lys185) in 10 µg of HEK293 nuclear extract.

    Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated dimethhyl G9a in 500 µg of HEK293 cell lysate.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to a region near the N-terminus of human G9a dimethylated at Lys185.
    表位 Near N-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes a region near the N-terminus of G9a methylated at Lys185. This antibody should recognizes isoform 1 (long form) and isoform 2 (short form) based upon immunogen design. This antibody demonstrates cross reactivity against monomethyl, and trimethyl G9a (Lys185).
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with Human. Predicted to react with Bovine, Canine, and Porcine based on 100% sequence homology. Other homologies: Rat and Mouse (90% sequence homology).
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_006700
    基因符号
    • NG36
    • EHMT2
    • BAT8
    • C6orf30
    • G9A
    • KMT1C
    纯化方法 Affinity purified
    UniProt编号
    • Q96KQ7
    产品使用声明
    质量保证 Evaluate by Western Blotting in HEK293 nuclear extract.

    Western Blotting Analysis: 2 µg/mL of this antibody detected dimethyl G9a (Lys185) in 10 µg of HEK293 nuclear extract.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,ABE489,Anti-dimethyl G9a Antibody (Lys185)

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