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merck millipore,默克密理博,05-1050X,Anti-Phosphotyrosine Antibody, 4G10 Platinum
产品名称:Anti-Phosphotyrosine Antibody, 4G10 Platinum
产品型号:05-1050X
Anti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA.
merck millipore,默克密理博,05-1050X,Anti-Phosphotyrosine Antibody, 4G10 Platinum
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A ELISA, WB, FC, IHC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-1050X 品牌系列 Upstate
商名 - Upstate
描述 Anti-Phosphotyrosine Antibody, 4G10® Platinum 背景信息 The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of. 产品信息 格式 Purified 控制 - Suggested Positive Antigen Control: Cat.# 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20ºC. The lysate is stable for 6 months at –20ºC.
演示 A proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2bκ) and PY20 (IgG2b) in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. 应用 应用 Anti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA. 主要应用 - ELISA
- Western Blotting
- Flow Cytometry
- Immunohistochemistry
- Immunocytochemistry
生物信息 免疫原品种 4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein. 克隆 4G10 宿主 Mouse 特异性 Tyrosine-phosphorylated proteins from all species 同种型 IgG2b 品种反应性 All 抗体类型 Monoclonal Antibody 修改 - Phosphorylation
纯化方法 Protein G-Sepharose™ chromatography 产品使用声明 质量保证 Routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.包装信息 数量 30 µL
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