merck millipore,默克密理博,17-10048,ChIPAb+ Histone H2A.Z

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merck millipore,默克密理博,17-10048,ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set
产品型号：17-10048
This ChIPAb+ Histone H2A.Z -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10048,ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10048,ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
H	WB, ChIP

描述
产品目录编号	17-10048
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Histone H2A.Z set includes the Histone H2A.Z antibody, a negative control antibody (normal sheep IgG), and qPCR primers which amplify a 166 bp region of human GAPDH. The Histone H2A.Z and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H2A.Z -associated chromatin.
Alternate Names	H2AZ histone H2A histone family, member Z
背景信息	H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

产品信息
格式	Affinity Purified
控制	Includes negative control sheep IgG antibody and primers specific for human GAPDH.
演示	Anti-Histone H2A.Z (Sheep polyclonal IgG). One vial containing 25 μg of affinity purified sheep polyclonal IgG in PBS, pH 7.4 containing 1% BSA with 0.02% sodium azide, before the addition of 30% glycerol. Store at -20°C. Normal Sheep IgG. One vial containing 25 µg of protein G purified sheep IgG in 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, with 0.01% sodium azide, before the addition of 30% glycerol. Store at -20°C. Control Primers. One vial containing 75 μL of 5 μM of each primer specific for human GAPDH. Store at -20°C. FOR: TAC TAG CGG TTT TAC GGG CG REV: TCG AAC AGG AGG AGC AGA GAG CGA

应用
应用	This ChIPAb+ Histone H2A.Z -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Western Blotting Chromatin Immunoprecipitation (ChIP)
应用说明	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Sheep IgG or 1 µg of Anti-Histone H2A.Z and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of Histone H2A.Z associated DNA fragments was verified by qPCR using Control Primers (Part No. 22-004) for a positive, and GAPDH Coding primers as a negative assay (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data HeLa lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Histone H2A.Z (1:1000 dilution). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

生物信息
免疫原品种	Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z.
表位	C-terminus
宿主	Sheep
特异性	Recognizes Histone H2A.Z, Mr ~15 kDa.
品种反应性	Human
Species Reactivity Note	Human. Wide species reactivity is expected. Predicted to cross-react with zebrafish, mouse, chicken, rat, rabbit, and Xenopus based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_036544
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene. [provided by RefSeq].
基因符号	H2AFZ H2A.z H2A/z H2AZ MGC117173
纯化方法	Affinity Purfied
UniProt编号	P0C0S5
UniProt汇总	FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Ref.7 SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP By similarity. SUBCELLULAR LOCATION:Nucleus. PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis By similarity. Not phosphorylated By similarity. SIMILARITY: Belongs to the histone H2A family. MOLECULAR WEIGHT: is 13413.4 Da from positions 2 - 128. Determined by ESI. Monoisotopic, not modified. Ref.8 Ref.10 Ref.11

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Sheep IgG or 1 µg of Anti-Histone H2A.Z and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of H2A.Z associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Sheep IgG or 1 µg of Anti-Histone H2A.Z and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of H2A.Z associated DNA fragments was verified by qPCR using Control Primers (Please see figures).

Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.