merck millipore,默克密理博,05-1050,4G10 Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A	FC, WB, ICC, ELISA, IHC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-1050
品牌系列	Upstate
商名	Upstate
描述	4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
背景信息	The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.

产品信息
格式	Purified
控制	Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
演示	Proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide. Liquid at 2-8°C.

应用
应用	4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry ELISA Immunohistochemistry
应用说明	Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.

生物信息
免疫原品种	4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
宿主	Mouse
特异性	Tyrosine-phosphorylated proteins from all species
同种型	IgG2b
品种反应性	All
抗体类型	Monoclonal Antibody
修改	Phosphorylation
纯化方法	Protein G-Sepharose™ chromatography

产品使用声明
质量保证	Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells. Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,05-1050,4G10 Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

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