merck millipore,默克密理愽
  • merck millipore,默克密理博,04-822,Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

  • merck millipore,默克密理博,04-822,Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

    产品名称：Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal
    产品型号：04-822
    This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).

    merck millipore,默克密理博,04-822,Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

  • 产品介绍
  • merck millipore,默克密理博,04-822,Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    H, RWBRbCulture SupernatantMonoclonal Antibody
    描述
    产品目录编号 04-822
    Replaces 05-822
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal
    Alternate Names
    • ras homolog
    背景信息 Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.
    产品信息
    格式 Culture Supernatant
    演示 Cultured supernantant in 0.05% sodium azide
    应用
    应用 This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).
    主要应用
    • Western Blotting
    应用说明 Western Blot Analysis:
    A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.
    生物信息
    免疫原品种 KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility
    克隆 3L74
    宿主 Rabbit
    特异性 Recognizes Rho (-A, -B, -C)
    同种型 IgG
    品种反应性 HumanRat
    Species Reactivity Note Broad species cross-reactivity expected based on sequence homology
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_001664
    基因符号
    • RHOA
    • ARH12
    • ARHA
    • H12
    • RHOH12
    • RhoA
    • Rho12
    • RHO12
    UniProt编号
    • P61586
    UniProt汇总 FUNCTION: SwissProt: P61586 # Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1.
    COFACTOR: Magnesium.
    SIZE: 193 amino acids; 21768 Da
    SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13.
    SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
    DOMAIN: SwissProt: P61586 The basic-rich region is essential for yopT recognition and cleavage.
    PTM: Substrate for botulinum ADP-ribosyltransferase. & Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
    SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
    产品使用声明
    质量保证 routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,04-822,Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

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下一件merck millipore产品：merck millipore,默克密理博,47-205,MILLIPLEX MAP HeLa Cell Lysate: Unstimulated - Lysates/Reagents for Multiplex Assays


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