merck millipore,默克密理愽
  • merck millipore,默克密理博,04-796,Anti-Akt1/PKBα Antibody, clone AW24,04-796

  • merck millipore,默克密理博,04-796,Anti-Akt1/PKBα Antibody, clone AW24,04-796

    产品名称：Anti-Akt1/PKBα Antibody, clone AW24, 04-796
    产品型号：04-796


    merck millipore,默克密理博,04-796,Anti-Akt1/PKBα Antibody, clone AW24,04-796

  • 产品介绍
  • merck millipore,默克密理博,04-796,Anti-Akt1/PKBα Antibody, clone AW24,04-796

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWBRbPurifiedMonoclonal Antibody
    描述
    产品目录编号 04-796
    Replaces 05-796
    描述 Anti-Akt1/PKBα Antibody, clone AW24 | 04-796
    Alternate Names
    • v-akt murine thymoma viral oncogene homolog 1
    • AKT1 kinase
    • Protein kinase B
    • RAC-alpha serine/threonine-protein kinase
    • rac protein kinase alpha
    背景信息 The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
    产品信息
    格式 Purified
    控制
    • HEK293 cell lysate.
    演示 Cultured supernantant containing 0.05% sodium azide.
    应用
    主要应用
    • Western Blotting
    生物信息
    免疫原品种 Sythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα.
    表位 C-terminus
    克隆 AW24
    浓缩 Cultured supernantant containing 0.05% sodium azide.
    宿主 Rabbit
    特异性 This antibody recognizes Akt1/PKBα.
    同种型 IgG
    品种反应性 RatHumanMouse
    Species Reactivity Note Human, mouse, rat.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_150233.1
    Entrez基因汇总 Inhibits JUN kinase activation and mediates inhibition of apoptosis.
    基因符号
    • RAC-PK-alpha
    • AKT
    • C-AKT
    • EC 2.7.11.1
    • MGC99656
    • PKB
    • PKB-ALPHA
    • PRKBA
    • RAC
    • RAC-ALPHA
    UniProt编号
    • P31749
    UniProt汇总 FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
    CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
    ENZYME REGULATION: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
    SUBUNIT STRUCTURE: Interacts with AGAP2 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN and THEM4. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression.
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.

    TISSUE SPECIFICITY: In all human cell types so far analyzed.
    DOMAIN: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane.The AGC-kinase C-terminal mediates interaction with THEM4.
    PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
    INVOLVEMENT IN DISEASE:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.
    Defects in AKT1 are associated with colorectal cancer (CRC).Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
    SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 protein kinase domain.
    产品使用声明
    质量保证 Evaluated by western blot on RIPA lysates from human HEK293 cells.
    Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Akt1/PKBα in RIPA lysates from human HEK293 cells.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µl

    merck millipore,默克密理博,04-796,Anti-Akt1/PKBα Antibody, clone AW24,04-796

上一件merck millipore产品：merck millipore,默克密理博,07-1514,Anti-phospho-WAVE2 (Ser351) Antibody
下一件merck millipore产品：merck millipore,默克密理博,14-480M,Rsk2/MAPKAP Kinase 1b Protein, active, 250 µg


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