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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5977 Replaces 03-114; 04-1041 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Musashi-1 Antibody Alternate Names - Musashi homolog 1
背景信息 In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi1 is a selective marker for intestinal stem or early lineage cells. Musashi1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi1 is present. 产品信息 格式 Affinity Purified 控制 - Mouse cortical neural stem cells (Catalog No. SCR029).
演示 Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. 应用 应用 Detect Musashi-1 using this Anti-Musashi-1 Antibody validated for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Immunocytochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Western blot:
1:200-1:1,000 using ECL.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide, amino acids 5-21 of Musashi. 宿主 Rabbit 特异性 Recognizes Musashi-1. 品种反应性 RatHumanMouse Species Reactivity Note Human and rodent. Reactivity with other species has not been confirmed. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002442.2
Entrez基因汇总 This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. 基因符号 - Musashi-1
- MSI1
纯化方法 Immunoaffinity purified UniProt编号 - O43347
UniProt汇总 FUNCTION: SwissProt: O43347 # RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
SIZE: 362 amino acids; 39125 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
DOMAIN: SwissProt: O43347 The first RNA recognition motif binds more strongly to RNA compared to the second one (By similarity).
SIMILARITY: Contains 2 RRM (RNA recognition motif) domains.产品使用声明 质量保证 Routinely evaluated by Western Blot on Human Placenta lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Musashi-1 on 10 μg of Human placenta lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -80ºC from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,S7813,CpG WIZ RASSF1A Amplification Kit
下一件merck millipore产品：merck millipore,默克密理博,71137,Rosetta-gami™ B(DE3)pLysS Competent Cells - Novagen
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