merck millipore,默克密理博,04-787,Anti-phospho-PKD (Ser916) Antibody, clone MC29, rabbit monoclonal

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H, H	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-787
Replaces	05-787
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-PKD (Ser916) Antibody, clone MC29, rabbit monoclonal
Alternate Names	Protein kinase D Protein kinase C mu type
背景信息	As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.

产品信息
格式	Purified
控制	RIPA lysates from HEK293 cells
演示	Cultured supernantant in 0.05% sodium azide

应用
应用	Detect phospho-PKD (Ser916) using this Anti-phospho-PKD (Ser916) Antibody, clone MC29 validated for use in WB.
主要应用	Western Blotting
应用说明	Western Blot Analysis: 1:500-1:2000 of this lot detected PKD in RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate (PDBu) for 10 minutes at 50 ng/mL.

生物信息
免疫原品种	peptide containing RVpSIL in which pS corresponds to phospho-serine 916 of mouse PKD
克隆	MC29
宿主	Rabbit
特异性	phospho-PKD
同种型	IgG
品种反应性	HumanHuman
Species Reactivity Note	Human. Predicted to cross-react with mouse based on sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_008858.3
Entrez基因汇总	Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
基因符号	PKD1 PRKD1 PKD PKCM nPKC-mu PRKCM PKC-mu nPKC-D1 PKC-MU
修改	Phosphorylation
UniProt编号	Q62101
UniProt汇总	FUNCTION: SwissProt: Q15139 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. SIZE: 912 amino acids; 101888 Da SUBUNIT: Interacts via its N-terminal region with CENTA1. PTM: Autophosphorylated (By similarity). SIMILARITY: SwissProt: Q15139 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 PH domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.

产品使用声明
质量保证	routinely evaluated by immunoblot on RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

包装信息
数量	100 µL

merck millipore,默克密理博,04-787,Anti-phospho-PKD (Ser916) Antibody, clone MC29, rabbit monoclonal

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