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品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, IHC R Purified Monoclonal Antibody 描述 产品目录编号 MAB5594 描述 Anti-HCN1 Antibody, clone RQT 7C3 Alternate Names - Hyperpolarization-activated and cyclic nucleotide-gated channel 1
背景信息 HCN1 and HCN2 play an important role for motor learning and synapse formation by integration of cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is high in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. Hyperpolarization activated cation channels of the HCN gene family such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore. 产品信息 格式 Purified 控制 - Mouse Brain Lysate
演示 Purified ascites in PBS with 0.05% NaN3 应用 应用 Anti-HCN1 Antibody, clone RQT 7C3 detects level of HCN1 & has been published & validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blot on rat and mouse retina. The antibody recognizes the ~110 kDa glycosylated protein from a retinal membrane preparation.
Immunohistochemistry on rat and mouse retina.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide from rat HCN1. 表位 C-Terminus 克隆 RQT 7C3 宿主 Rat 特异性 HCN1 (Hyperpolarization-activated and cyclic nucleotide-gated channel 1). The antibody recognizes both the glycosyated and deglycosylated form of HCN1. 同种型 IgG1 品种反应性 RatMouse Species Reactivity Note Mouse and Rat. Reactivity with other species has not been determined. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_021072.2
Entrez基因汇总 Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM] 基因符号 - HAC-2
- HCN1
- BCNG1
- BCNG-1
纯化方法 PBS UniProt编号 - Q9JKB0
UniProt汇总 FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP (By similarity). May mediate responses to sour stimuli.
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2 (By similarity). Interacts with KCNE2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in cerebral cortex, cerebellum, throughout the hippocampus, in medial habenula, anterior dorsal nucleus in the thalamus, tenia tecta, several nuclei of the general motor system and in optic nerve layer. Detected in a subset of elongated cells in taste buds.
DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel HCN family.
SIMILARITY: Contains 1 cyclic nucleotide-binding domain.
产品使用声明 质量保证 Routinely evaluated by western blot on mouse brain lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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