merck millipore,默克密理博,04-1151,Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32, rabbit monoclonal

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merck millipore,默克密理博,04-1151,Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32, rabbit monoclonal
产品名称：Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32, rabbit monoclonal
产品型号：04-1151
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1151,Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1151,Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1151
描述	Anti-Cyclin D1 Antibody, clone EPR2241(IHC)-32, rabbit monoclonal
Alternate Names	cyclin D1 (PRAD1: parathyroid adenomatosis 1) B-cell CLL/lymphoma 1 BCL-1 oncogene G1/S-specific cyclin D1 PRAD1 oncogene cyclin D1
背景信息	Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.

产品信息
格式	Unpurified
控制	MCF-7 cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Cyclin D1 in human mantle cell lymphoma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC. Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the C-terminus of human Cyclin D1.
表位	C-terminus
克隆	EPR2241(IHC)-32
宿主	Rabbit
特异性	This antibody recognizes Cyclin D1.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_444284.1
Entrez基因汇总	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
基因符号	U21B31 BCL1 D11S287E PRAD1
纯化方法	Unpurified
UniProt编号	P24385
UniProt汇总	FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition. SUBUNIT STRUCTURE: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. PTM: Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. INVOLVEMENT IN DISEASE: A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus. SEQUENCE SIMILARITIES: Belongs to the cyclin family. Cyclin D subfamily.

产品使用声明
质量保证	Evaluated by Western Blot on MCF-7 cell lysates. Western Blot Analysis: A 1:10,000-50,000 dilution of this antibody was used to detect Cyclin D1 in MCF-7 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.