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merck millipore,默克密理博,04-1091,Anti-Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal
产品名称：Anti-Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal
产品型号：04-1091
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
merck millipore,默克密理博,04-1091,Anti-Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Unpurified Monoclonal Antibody 描述 产品目录编号 04-1091 描述 Anti-Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal Alternate Names - sirtuin type 2
- SIR2-like protein 2
- silencing information regulator 2-like
- silent information regulator 2
- sir2-related protein type 2
- sirtuin (silent mating type information regulation 2
- homolog) 2 (S. cerevisiae)
- sirtuin (silent mating type information regulation 2,
- S.cerevisiae, homolog) 2
- sirtuin 2
背景信息 Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein. 产品信息 格式 Unpurified 控制 - Jurkat cell lysate
演示 Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Sir2 in human colon and lung squamous carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.生物信息 免疫原品种 Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2. 表位 C-terminus 克隆 E104 宿主 Rabbit 特异性 The antibody does not cross-react with other sirtuin family members. 同种型 IgG 品种反应性 Human Species Reactivity Note Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001135970.1
Entrez基因汇总 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. 基因符号 - SIR2L2
- EC 3.5.1.-
- SIR2
- SIR2-like
- SIR2L
纯化方法 Unpurified UniProt编号 - Q8IXJ6
UniProt汇总 FUNCTION: NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.
CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.
COFACTOR: Binds 1 zinc ion per subunit.
ENZYME REGULATION: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.
SUBUNIT STRUCTURE: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules.
TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
DEVELOPMENTAL STAGE: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
PTM: Phosphorylated at the G2/M transition of the cell cycle.
SEQUENCE SIMILARITIES: Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
SEQUENCE CAUTION: The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.产品使用声明 质量保证 Evaluated by Western Blot on Jurkat cell lysates.
Western Blot Analysis: A 1:5,000 dilution of this antibody was used to detect Sir2 in Jurkat cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. 包装信息 数量 100 µL
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