merck millipore,默克密理博,04-1046,Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit,04-1046

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merck millipore,默克密理博,04-1046,Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit,04-1046
产品名称：Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit, 04-1046
产品型号：04-1046
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, R More>>

merck millipore,默克密理博,04-1046,Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit,04-1046

产品介绍

merck millipore,默克密理博,04-1046,Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit,04-1046

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	IHC, WB, ICC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-1046
描述	Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit \| 04-1046
Alternate Names	neurogenic locus notch homolog protein 1 Translocation-associated notch protein TAN-1
背景信息	Notch is the receptor for Delta and Jagged ligands, which cause proteolysis of notch to liberate the intracellular domain. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved in numerous differentiation and cell lineage determination steps.

产品信息
格式	Purified
控制	Hek293 cell lysates
演示	Rabbit monoclonal IgG in buffer containing 50mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Notch1 (Cytoplasmic) Antibody, clone EP1238Y, Rabbit detects level of Notch1 (Cytoplasmic) & has been published & validated for use in WB, IC, IH.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Immunohistochemistry Analysis: 1:50 - 100 dilution from a previous lot detected Notch1 in Human fetal lung tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to the cytoplasmic domain of human Notch1
表位	Cytoplasmic Domain
克隆	EP1238Y
宿主	Rabbit
特异性	This antibody recognizes the cytoplasmic domain of Notch1
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_060087.3
Entrez基因汇总	This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq]
基因符号	notch1 TAN1 hN1
UniProt编号	P46531
UniProt汇总	FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia By similarity. SUBUNIT STRUCTURE: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. Ref.6 Ref.7 Ref.8 SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane proteinBy similarity. Notch 1 intracellular domain: NucleusBy similarity. Note= Following proteolytical processing NICD is translocated to the nucleus By similarity. TISSUE SPECIFICTY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane By similarity. Phosphorylated By similarity. INVOLVEMENT IN DISEASE: NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. Ref.13 Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population. SEQUENCE SIMILARITIES: Belongs to the NOTCH family. Contains 5 ANK repeats. Contains 36 EGF-like domains. Contains 3 LNR (Lin/Notch) repeats.

产品使用声明
质量保证	Evaluated by Western Blotting on HEK293 cell lysates. Western Blotting Analysis: 1:500 dilution of this antibody detected Notch1 in HEK293 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.