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品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, R, Fe, Ca, Po WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9568 Replaces 04-1112 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament L Antibody 背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10 nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. 产品信息 格式 Serum 控制 - Brain tissue
演示 Rabbit serum. Contains no preservative. 应用 应用 Detect Neurofilament L using this Anti-Neurofilament L Antibody validated for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western Blot Analysis:
1:1000 dilution of a previous lot detected NEUROFILAMENT L on 10 μg of mouse brain lysates.
Immunocytochemistry:
A 1:200-1:500 dilution of a previous lot was used in IC.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Purified porcine NF-L 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Neurofilament-Light (NF-L) 品种反应性 BovineHumanMouseRatFelineCaninePig Species Reactivity Note Human, rat, mouse, feline, canine, porcine and bovine. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006158.2
基因符号 - NFL
- NEFL
- CMT1F
- NF-L
- NF68
- CMT2E
纯化方法 Unpurified UniProt编号 - P07196
UniProt汇总 FUNCTION: SwissProt: P07196 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
SIZE: 543 amino acids; 61517 Da
DOMAIN: SwissProt: P07196 The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
PTM: O-glycosylated (By similarity).
DISEASE: SwissProt: P07196 # Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
SIMILARITY: Belongs to the intermediate filament family.
MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.产品使用声明 质量保证 Immunohistochemistry(paraffin) Analysis:
Neurofilament-L (cat. # AB9568) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:800, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) around a Purkinje cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Human Cerebellum使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 50 µL
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下一件merck millipore产品：merck millipore,默克密理博,ABE601,Anti-CPEB1 Antibody
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