merck millipore,默克密理博,04-1039,Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1039,Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal
产品名称：Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal
产品型号：04-1039
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Ras/p21 Antibody, clone EP1125Y, Rabbit is More>>

merck millipore,默克密理博,04-1039,Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1039,Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1039
描述	Anti-Ras/p21 Antibody, clone EP1125Y, rabbit monoclonal
Alternate Names	triphosphatase-activating protein GTPase-activating protein RAS p21 protein activator (GTPase activating protein) 1 RAS p21 protein activator 1 Ras p21 protein activator
背景信息	Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and Ral GDS.

产品信息
格式	Unpurified
控制	C6 cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Ras/p21 Antibody, clone EP1125Y, Rabbit is validated for use in WB, FC, IP for the detection of Ras/p21.
主要应用	Flow Cytometry Western Blotting
应用说明	Flow Cytometry Analysis: Flow cytometric analysis of permeabilized PC-12 cells using Anti-Ras (1:100 dilution). Immunoprecipitation: A 1:30 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminal region of human Ras.
表位	N-terminus
克隆	EP1125Y
宿主	Rabbit
特异性	This antibody detects H-Ras, N-Ras and K-Ras at and around the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002515.1
Entrez基因汇总	This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML).
基因符号	p120RASGAP CM-AVM CMAVM DKFZp434N071 GAP PKWS RASA RASGAP RasGAP p120GAP
纯化方法	Unpurified
UniProt编号	P20936
UniProt汇总	FUNCTION: Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. SUBUNIT STRUCTURE: Interacts with SQTSM1. Interacts with SPSB1. Interaction with SPSB1 does not promote degradation. SUBCELLULAR LOCATION: Cytoplasm. PTM: The N-terminus is blocked. INVOLVEMENT IN DISEASE: Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas. Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. SEQUENCE SIMILARITIES: Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.

产品使用声明
质量保证	Evaluated by Western Blot on C6 cell lysates. Western Blot Analysis: A 1:500,000 dilution of this antibody was used to detect Ras in C6 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.