merck millipore,默克密理博,MABC135,Anti-ATG12 Antibody, clone EPR4800, rabbit monoclonal

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merck millipore,默克密理博,MABC135,Anti-ATG12 Antibody, clone EPR4800, rabbit monoclonal
产品名称：Anti-ATG12 Antibody, clone EPR4800, rabbit monoclonal
产品型号：MABC135
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-ATG12 Antibody, clone EPR4800, Rabbit is an ant More>>

merck millipore,默克密理博,MABC135,Anti-ATG12 Antibody, clone EPR4800, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABC135,Anti-ATG12 Antibody, clone EPR4800, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABC135
描述	Anti-ATG12 Antibody, clone EPR4800, rabbit monoclonal
Alternate Names	APG4 autophagy 4 homolog A ATG4 autophagy related 4 homolog A (S. cerevisiae) Autophagy-related cysteine endopeptidase 2 Autophagy-related protein 4 homolog A AUT-like 2 cysteine endopeptidase AUT-like 2, cysteine endopeptidase (S. cerevisiae) APG4 autophagy 4 homolog A (S. cerevisiae) autophagin 2 AUT-like 2, cysteine endopeptidase autophagin-2 cysteine protease ATG4A
背景信息	Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).

产品信息
格式	Diluted Culture Supernatant
控制	Fetal kidney tissue lysate, HepG2 and Raji cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-ATG12 Antibody, clone EPR4800, Rabbit is an antibody against ATG12 for use in WB, IH(P) & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC. Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected ATG12 in human breast carcinoma tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to human ATG12 at the C-terminus.
表位	C-terminus
克隆	EPR4800
宿主	Rabbit
特异性	This antibody recognizes ATG12 at the C-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human. Predicted to react with, mouse and rat based on 100% sequence homology.
抗体类型	Monoclonal Antibody
基因符号	Autophagin-2 APG4A AUTL2 hAPG4A OTTHUMP00000023825 EC 3.4.22.-
纯化方法	Unpurified
UniProt编号	O94817
UniProt汇总	FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm Probable. TISSUE SPECIFICITY: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. SEQUENCE SIMILARITIES: Belongs to the peptidase C54 family.

产品使用声明
质量保证	Evaluated by Western Blot in fetal kidney tissue lysate, HepG2 and Raji cell lysates. Western Blot Analysis: A 1:1,000-10,000 dilution of this antibody detected ATG12 in Fetal kidney tissue lysate, HepG2 and Raji cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.