merck millipore,默克密理博,03-115,RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal

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merck millipore,默克密理博,03-115,RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal
产品名称：RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal
产品型号：03-115
This RIPAb+ Musashi 2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-115,RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal

产品介绍

merck millipore,默克密理博,03-115,RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal

重要规格表

品种反应性	主要应用
R, H, M	FC, WB

描述
产品目录编号	03-115
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Alternate Names	musashi homolog 2 (Drosophila) musashi 2
背景信息	In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. MSI2 or Musashi2 is a member of an RNA-binding protein family that appears to play a central role in post transcriptional gene regulation. The MSI2 gene encodes a protein containing two conserved tandem RNA recognition motifs. MSI2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages.

产品信息
格式	Unpurified
控制	Includes negative control rabbit IgG antibody and control primers specific for human PTP4A1.
演示	Anti-Musashi 2 (Rabbit Monoclonal). One vial containing 50 μL of Rabbit Monoclonal Antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C. Normal Rabbit IgG. One vial containing 125 μg of Rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, PTP4A1. One vial containing 75 μL of 5 μM of each primer specific for human PTP4A1. Store at -20°C. FOR: ATC CAA CCA ATG CGA CCT TA REV: AAG GCC AAT CAA GAA CAT GG

应用
应用	This RIPAb+ Musashi 2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Flow Cytometry Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 2 antibody. Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 2) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 2 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit. Arrow indicates Musashi 2 (Please see figures). Immunohistochemistry (Paraffin) Analysis: Representative lot data. A previous lot was used in immunohistochemical staining of paraffin-embedded human placenta tissue using 1:100-1:250 dilution of anti-Musashi 2 (Please see figures). Western Blot Analysis: Representative lot data. A 1:1,000-2,000 dilution of a previous lot of this antibody was used to detect Musashi 2 in human brain (A), rat brain (B), SW480 (C), and T47D (D) cell lysates (Please see figures). Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.

生物信息
免疫原品种	Synthetic peptide corresponding to N-terminus of human Musashi-2.
表位	N-terminus
宿主	Rabbit
特异性	Recognizes Musashi-2 at and around the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_620412.1
Entrez基因汇总	This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.
基因符号	Musashi-2 FLJ36569 MGC3245 MSI2H
纯化方法	Unpurified
UniProt编号	Q96DH6
UniProt汇总	FUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with polysomes By similarity. TISSUE SPECIFICITY: Ubiquitous; detected at low levels. INDUCTION: Up-regulated in astrocytes after brain injury By similarity. PTM: Phosphorylated By similarity. INVOLVEMENT IN DISEASE: Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). SEQUENCE SIMILARITIES: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Musashi 2-associated RNA was verified by qPCR using RIP Primers PTP4A1 (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Musashi 2-associated RNA was verified by qPCR using RIP Primers PTP4A1 (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.