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主要应用 品种 来源 CULT Human Recombinant human DNA expressed in baculovirus infected Sf9 cells 描述 产品目录编号 01-185 品牌系列 Upstate
商名 - Upstate
描述 VEGF Protein, Human recombinant 产品信息 配方 演示 25mM HEPES and 150mM NaCl, pH 7.0. 应用 应用 The human recombinant VEGF Protein is available in a 10 µg format. 主要应用 - Cell Culture
应用说明 VEGF Proliferation Assay
1. Prior to use in this assay, maintain HUVE cells in F-12K medium supplemented with 10% (v/v) FBS, 1% penicillin-streptomycin-glutamine, 0.1 mg/ml heparin and 0.05 mg/ml endothelial cell growth supplement (ECGS).
2. For assay of VEGF, subculture HUVE cells into a 96-well plate at a density of 4,000 cells per well in the growth medium over night.
3. Add VEGF to a final concentration range of 0.2, 0.4, 0.8, 1.6, 3.2, 6.2, 12.5, 25 and 50ng/ml. Add VEGF diluent to one set of wells as the negative control.
4. Four days later, add 20ul of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well.
5. Incubate at 37C for 2 hrs and read at OD 490nm.生物信息 来源 Recombinant human DNA expressed in baculovirus infected Sf9 cells 品种 Human Entrez基因编号 - NM_003376.4
- NM_001025366.1
- NM_001025367.1
- NM_001025368.1
- NM_001025369.1
- NM_001025370.1
- NM_001033756.1
Entrez基因汇总 This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. 基因符号 - MGC70609
- VEGFA
- VEGF-A
- VEGF
- VPF
物理形式 Frozen liquid 纯化方法 Sterilized through a 0.2 micron membrane filter UniProt编号 - P15692
UniProt汇总 FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
SIZE: 232 amino acids; 27042 Da
SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.产品使用声明 质量保证 Routinely evaluated in a 5-day growth assay using human umbilical vein endothelial cells 质量控制测试 Cell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 2 years at -70°C from time of shipment. Aliquot to avoid repeated freezing and thawing. : This product is acid stable. Dilute in either 0.1% TFA or 4mM HCl, containing 25μg BSA per g VEGF to maintain stability. Reducing agents (such as dithiothreitol or 2-mercaptoethanol) inhibit VEGF activity. 包装信息 数量 10 µg
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