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品种反应性 主要应用 宿主 格式 抗体类型 R, M, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN78 描述 Anti-NeuN (rabbit) Antibody Alternate Names - NeuN
- NEUronal Nuclei
- clone A60
背景信息 ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. 产品信息 格式 Affinity Purified 控制 - Mouse brain nuclear extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-NeuN (rabbit) Antibody is validated for use in WB, IH(P), IC for the detection of NeuN (rabbit). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected NeuN in normal mouse hippocampus and normal mouse midbrain tissues.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NeuN in rat E18 cortex/hippocampus primary cultures.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to mouse NeuN. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes NeuN at the N-terminus. 品种反应性 RatMouseHuman Species Reactivity Note Demonstrated to react with Mouse, Human, and Rat. 抗体类型 Polyclonal Antibody 基因符号 - NeuN
纯化方法 Affinity purified 产品使用声明 质量保证 Evaluated by Western Blot in mouse brain nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,539122,Protamine Sulfate, Salmon Milt
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