merck millipore,默克密理博,TR-1008-G,Sodium Butyrate Solution

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merck millipore,默克密理博,TR-1008-G,Sodium Butyrate Solution
产品名称：Sodium Butyrate Solution
产品型号：TR-1008-G
This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.

merck millipore,默克密理博,TR-1008-G,Sodium Butyrate Solution

产品介绍

merck millipore,默克密理博,TR-1008-G,Sodium Butyrate Solution

描述
产品目录编号	TR-1008-G
商名	Specialty Media
描述	Sodium Butyrate Solution
概述	This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.

应用
应用	This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.
应用说明	Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert. This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.

应用

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.

应用说明

Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.

This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.