merck millipore,默克密理博,SCR080,MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,SCR080,MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
产品名称：MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
产品型号：SCR080
MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit provides ready-to use primary rat neural stem cells that are constitutively labeled with hmGFP along with expansion medi More>>

merck millipore,默克密理博,SCR080,MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

产品介绍

merck millipore,默克密理博,SCR080,MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

描述
产品目录编号	SCR080
商名	Chemicon MilliTrace
描述	MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
概述	MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit provides ready-to use primary rat neural stem cells that are constitutively labeled with the humanized mulleri green fluorescent protein (hmGFP) along with expansion medium to help maintain expression of the transgene. hmGFP expression is driven off a constitutive promoter, chicken actin. These stable GFP transfected cells have been FACS sorted to over 95-100% purity. The constitutive GFP Reporter Adult Rat Hippocampal NSCs display the immunochemical staining properties of NSC; they are Nestin+Sox2+TuJ1-GFAP-. These cells are multipotent. Under defined neuronal differentiation conditions (Rodent Neuron Differentiation Kit, Millipore Cat. No. SCR035), the majority of cells are differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+GFAP-). Under defined astrocytic differentiation conditions (Astrocyte Differentiation Medium, Millipore Cat. No. SCM010), majority of cells differentiate to GFAP-positive astrocytes (GFP+GFAP+βIII-tubulin-).

产品信息
部件	MilliTrace Rat Neural Stem Cell Expansion Medium (Cat. No. SCM040) MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells, 1 x 10⁶ viable cells

应用
应用	MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit provides ready-to use primary rat neural stem cells that are constitutively labeled with hmGFP along with expansion medium to help maintain expression of the transgene.

生物信息
细胞系类型	Neural Stem Cells

产品使用声明
质量保证	Primary constitutive GFP reporter rat neural stem cells have been validated for high level of constitutive expression of GFP, high level of expression of Nestin and Sox 2, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma.
使用声明	For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

质量保证

Primary constitutive GFP reporter rat neural stem cells have been validated for high level of constitutive expression of GFP, high level of expression of Nestin and Sox 2, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma.

使用声明

For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

储存和货运信息
存储条件	· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Part No. SCC080) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages. · Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating. · 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years. · Lyophilized FGF-2 (Part No. GF003-10UG) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.

储存和货运信息

存储条件

· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Part No. SCC080) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages.
· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating.
· 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
· Lyophilized FGF-2 (Part No. GF003-10UG) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.

包装信息
数量	1 kit

merck millipore,默克密理博,SCR080,MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

上一件merck millipore产品：merck millipore,默克密理博,AB1837P,Anti-Tumor Necrosis Factor-α Antibody
下一件merck millipore产品：merck millipore,默克密理博,14-640M,JAK2 Protein, active, 250 µg

merck millipore,默克密理博,324744,Elastase Inhibitor II - Calbiochem

merck millipore,默克密理博,AP200,Goat Anti-Mouse light chain Antibody

merck millipore,默克密理博,AB5832,Anti-BACE Antibody

merck millipore,默克密理博,AB1837P,Anti-Tumor Necrosis Factor-α Antibody

merck millipore,默克密理博,14-640M,JAK2 Protein, active, 250 µg

merck millipore,默克密理博,8410-OP,OmniPur 20X SSPE Buffer Liquid Concentrate - Calbiochem

merck millipore,默克密理博,540221,Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

merck millipore,默克密理博,CC119,Human Caspase 3, recombinant human active

merck millipore,默克密理博,04-1005,Anti-FKHR Antibody, clone EP927Y, rabbit monoclonal

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech

merck millipore,默克密理博,SCR080,MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

重要规格表