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merck millipore,默克密理博,04-1005,Anti-FKHR Antibody, clone EP927Y, rabbit monoclonal
产品名称：Anti-FKHR Antibody, clone EP927Y, rabbit monoclonal
产品型号：04-1005
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
merck millipore,默克密理博,04-1005,Anti-FKHR Antibody, clone EP927Y, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Unpurified Monoclonal Antibody 描述 产品目录编号 04-1005 描述 Anti-FKHR Antibody, clone EP927Y, rabbit monoclonal Alternate Names - forkhead, Drosophila, homolog of, in rhabdomyosarcoma
- Forkhead box protein O1A
- Forkhead in rhabdomyosarcoma
- forkhead box O1
- forkhead box O1A (rhabdomyosarcoma)
- forkhead homolog in rhabdomyosarcoma
背景信息 The Non-Radioactive FoxO1 Transcription Factor Assay kit is provided in a 96-well format. During the assay, the Capture Probe, a double stranded biotinylated oligonucleotide containing the consensus sequence for FoxO1 binding (5'-TTGTTTAC-3'), is mixed with whole cell or nuclear extract in the Transcription Factor Assay Buffer provided directly in the Streptavidin coated plate. When incubated together, FoxO1 contained in the nuclear extract binds to its consensus sequence. The biotinylated double stranded oligonucleotide bound to FoxO1 protein is immobilized and any inactive, unbound material is washed away. The bound FoxO1 transcription factor is detected with a specific rabbit anti-FoxO1, primary antibody. An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader. Included in the kit are positive control whole cell extract, a non-specific double stranded oligonucleotide, and a specific double stranded competitor oligonucleotide. 产品信息 格式 Unpurified 控制 - NIH/3T3 cell lysate
演示 Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected FKHR in human bladder carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected FKHR in HeLa cells.生物信息 免疫原品种 Synthetic peptide corresponding to residues surrounding Ser329 of human FKHR. 表位 Ser329 克隆 EP927Y 宿主 Rabbit 特异性 This antibody recognizes both phosphorylated and unphosphorylated FKHR. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002015.3
基因符号 - OTTHUMP00000018301
- FKH1
- FKHR
- FOXO1A
纯化方法 Unpurified UniProt编号 - Q12778
UniProt汇总 FUNCTION: Transcription factor. Ref.9
SUBUNIT STRUCTURE: Interacts with LRPPRC By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Phosphorylated by AKT1; insulin-induced By similarity. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
INVOLVEMENT IN DISEASE: Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.产品使用声明 质量保证 Evaluated by Western Blot on NIH/3T3 cell lysates.
Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect FKHR in NIH/3T3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 100 µL
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