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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1837P Replaces 04-1114 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tumor Necrosis Factor-α Antibody Alternate Names - TNF-alpha
产品信息 格式 Affinity Purified 演示 Affinity Purified Immunoglobulin. Lyophilized from PBS pH 7.2. Contains no preservative. Reconstitute with sterile distilled water. 应用 应用 Anti-Tumor Necrosis Factor-α Antibody is an antibody against Tumor Necrosis Factor-α for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of
0.2-0.4 ng/well when rat TNF-α is used to coat the plate.
Western blot: 0.1 - 0.2 μg/mL, this dilution should offer detection limit of 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Highly purified (>98%) recombinant rat TNF-alpha 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Reacts with rat TNF-alpha 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000594.2
Entrez基因汇总 This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. 基因符号 - TNF-a
- TNF
- TNFSF2
- Cachectin
- cachectin
- DIF
- TNF-alpha
- OTTHUMP00000037669
- TNFA
UniProt编号 - P01375
UniProt汇总 FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
SIZE: 233 amino acids; 25644 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C. 包装信息 数量 50 µg
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