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merck millipore,默克密理博,SCR017,Pancreatic Cell Cryopreservation Medium
产品名称:Pancreatic Cell Cryopreservation Medium
产品型号:SCR017
The Pancreatic Cell Cryopreservation Medium (2X) is intended for the cryopreservation of LT2 Immortalized Pancreatic Mesenchymal Cells (SCR013) & VIT1 Primary Pancreatic Mesenchymal Cells (SCR014).
merck millipore,默克密理博,SCR017,Pancreatic Cell Cryopreservation Medium
- 产品介绍
重要规格表
主要应用 CULT 描述 产品目录编号 SCR017 品牌系列 Chemicon®
商名 - Chemicon
描述 Pancreatic Cell Cryopreservation Medium 概述 Pancreatic Cell Cryopreservation Medium (2X) is intended for the cryopreservation of LT2 Immortalized Pancreatic Mesenchymal Cells (SCR013) and VIT1 Primary Pancreatic Mesenchymal Cells (SCR014). 应用 应用 The Pancreatic Cell Cryopreservation Medium (2X) is intended for the cryopreservation of LT2 Immortalized Pancreatic Mesenchymal Cells (SCR013) & VIT1 Primary Pancreatic Mesenchymal Cells (SCR014). 主要应用 - Cell Culture
应用说明 PROCEDURES:
Reagents & Equipment required:
· Pancreatic Cell Cryopreservation Medium (SCR017)
· Cryogenic vials
1. Add an equal volume of Pancreatic Cell Cryopreservation Medium (2X) to a known volume of cell suspension in RPMI 1640 containing 25 mM HEPES, pH 7.2. It is preferable to freeze LT2 or VIT1 cells at a concentration of 2 to 5 million cells per mL.
2. Dispense into cryogenic vials using suitable volumes for the desired applications.
3. Freeze the cell suspension at a slow rate, approximately 1°C/minute by standard methods.
4. After complete freezing, transfer vials of cells to liquid N2 containing Dewar flask preferably in the vapor phase for long-term storage at maximum viability生物信息 媒质形式 Liquid 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The Pancreatic Cell Culture Supplement concentrate is provided frozen. Prior to use, thaw the entire bottle at room temperature or at refrigerator temperature overnight. Aliquot and store according to usage needs. The concentrate is stable until expiration (2 years from date of manufacture) at -20°C or for one month at 4°C. Three 10 mL aliquots are useful for most applications, with two aliquots maintained at -20°C . 包装信息 数量 30 mL 包装 2X concentrate
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