merck millipore,默克密理博,SCM041,MilliTrace Mouse Neural Stem Cell Expansion Medium

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merck millipore,默克密理博,SCM041,MilliTrace Mouse Neural Stem Cell Expansion Medium
产品名称：MilliTrace Mouse Neural Stem Cell Expansion Medium
产品型号：SCM041
MilliTrace Mouse Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter mouse neural stem cells.

merck millipore,默克密理博,SCM041,MilliTrace Mouse Neural Stem Cell Expansion Medium

产品介绍

merck millipore,默克密理博,SCM041,MilliTrace Mouse Neural Stem Cell Expansion Medium

重要规格表

主要应用
Cult

描述
产品目录编号	SCM041
商名	Chemicon
描述	MilliTrace Mouse Neural Stem Cell Expansion Medium
概述	MilliTrace Mouse Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter mouse neural stem cells. The Neural Stem Cell Basal Medium is a defined serum-free medium that helps support the growth and in vitro differentiation of neural stem cells derived from rodents. Basic Fibroblast Growth Factor (FGF-2, bFGF), Epidermal Growth Factor (EGF), and Heparin are provided separately, which when added to the Neural Stem Cell Basal Medium (SCM003) allow for the growth and proliferation of mouse neural stem cells. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells. Puromycin Solution is provided separately to help maintain the expression of the GFP labeled transgene.

产品信息
部件	Puromycin Solution, 100 µL Neural Stem Cell Basal Medium, 500 mL Basic FGF, 10 µg EGF Solution, 100 µL Heparin Solution, 150 µL

应用
应用	MilliTrace Mouse Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter mouse neural stem cells.
主要应用	Stem Cell Culture

储存和货运信息
存储条件	· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month. · Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity. · EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity. · 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.

储存和货运信息

存储条件

· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
· Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
· EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
· 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.