merck millipore,默克密理博,S7842,CpG WIZ H19-IGF2 Amplification Kit

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Merck Millipore 默克密理愽

描述
产品目录编号	S7842
品牌系列	Chemicon®
商名	Chemicon CpG Wiz
描述	CpG WIZ® H19-IGF2 Amplification Kit
概述	Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992 ). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). Aberrant methylation of H19-IGF2 locus is implicated in several inherited genetic disorders. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

产品信息
部件	Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C The components of the CpG WIZ® H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls. U Primer Set7.5 μM each primer (25X) 35 μL (clear cap) 90595 -15°C to -25°C M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90596 -15°C to -25°C W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90597 -15°C to -25°C U control DNA0.1 μg/μL 50 μL (clear cap) 90393 -15°C to -25°C M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

产品信息

部件

Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C
The components of the CpG WIZ® H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
U Primer Set7.5 μM each primer (25X) 35 μL (clear cap) 90595 -15°C to -25°C
M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90596 -15°C to -25°C
W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90597 -15°C to -25°C
U control DNA0.1 μg/μL 50 μL (clear cap) 90393 -15°C to -25°C
M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

应用
应用	The components of the CpG WIZ H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples.
主要应用	PCR
应用说明	Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® H19-IGF2 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

生物信息
品种反应性	Human
Entrez基因编号	NM_001007139.3 NM_000612.3
Entrez基因汇总	This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
基因符号	Somatomedin-A IGF2 ORFNames=PP1446 INSIGF FLJ22066 FLJ44734 pp9974 C11orf43 IGF-II
UniProt编号	P01344
UniProt汇总	FUNCTION: SwissProt: P01344 # The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. SIZE: 180 amino acids; 20140 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P01344 ## Belongs to the insulin family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.