merck millipore,默克密理博,S7803,CpG WIZ MGMT

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Merck Millipore 默克密理愽

merck millipore,默克密理博,S7803,CpG WIZ MGMT - Methylation specific PCR assay

描述
产品目录编号	S7803
品牌系列	Chemicon®
商名	Chemicon CpG Wiz
描述	CpG WIZ® MGMT - Methylation specific PCR assay
概述	Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). O(6)-Methylguanine DNA methyltransferase (MGMT) is an example of a gene that exhibits characteristic hypermethylation. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

产品信息
部件	Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C The components of the CpG WIZ® MGMT Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls. U Primer Set5 μM each primer (25X) 35 μL (neutral cap) 90513 -15°C to -25°C M Primer Set5 μM each primer (25X) 35 μL (red cap) 90514 -15°C to -25°C W Primer Set5 μM each primer (25X) 35 μL (green cap) 90515 -15°C to -25°C U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

产品信息

部件

Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C
The components of the CpG WIZ® MGMT Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
U Primer Set5 μM each primer (25X) 35 μL (neutral cap) 90513 -15°C to -25°C
M Primer Set5 μM each primer (25X) 35 μL (red cap) 90514 -15°C to -25°C
W Primer Set5 μM each primer (25X) 35 μL (green cap) 90515 -15°C to -25°C
U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C
M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

应用
应用	The components of the CpG WIZ MGMT Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
主要应用	PCR
应用说明	Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® MGMT Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

生物信息
品种反应性	Human
Entrez基因编号	NM_002412.2
基因符号	EC 2.1.1.63 MGMT
UniProt编号	P16455
UniProt汇总	FUNCTION: SwissProt: P16455 # Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. COFACTOR: Binds 1 zinc ion. SIZE: 207 amino acids; 21646 Da SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P16455 ## Belongs to the MGMT family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.