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merck millipore,默克密理博,MAB1794,Anti-Human B cells (CD19) Antibody, clone FMC63
产品名称：Anti-Human B cells (CD19) Antibody, clone FMC63
产品型号：MAB1794
This Anti-Human B cells (CD19) Antibody, clone FMC63 is validated for use in FC, IF for the detection of CD19.
merck millipore,默克密理博,MAB1794,Anti-Human B cells (CD19) Antibody, clone FMC63
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1794 Replaces CBL141 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Human B cells (CD19) Antibody, clone FMC63 概述 BIOCHEMISTRY:
p I = 7.0Alternate Names - B-lymphocyte Surface Antigen B4
产品信息 格式 Purified 演示 Purified from mouse ascitic fluid. The antibody is supplied in 25 mM Tris-HCl buffer, 0.4 M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry. 应用 应用 This Anti-Human B cells (CD19) Antibody, clone FMC63 is validated for use in FC, IF for the detection of CD19. 主要应用 - Flow Cytometry
应用说明 This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.
SUGGESTED USAGE DILUTION
Flow cytometry and Indirect Immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.生物信息 免疫原品种 Raji cells 克隆 FMC63 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.
Cell reactivity:
Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.
Clinical:
B cell chronic lymphocytic leukemia (B-CLL) +
Pro lymphocytic leukemia (PLL) +
Hairy cell leukemia (HCL) +
Common acute lymphoblastic leukemia (CALL) +
Pre-B acute lymphoblastic leukemia (pre-B-ALL) +
NULL-acute lymphoblastic leukemia (NULL-ALL) +
T common lymphocytic leukemia (T-CLL) -同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001770.4
Entrez基因汇总 Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. 基因符号 - Leu-12
- CD19
- B4
- MGC12802
UniProt编号 - P15391
UniProt汇总 FUNCTION: SwissProt: P15391 # Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
SIZE: 556 amino acids; 61128 Da
SUBUNIT: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:SwissProt: P15391 # Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].
SIMILARITY: SwissProt: P15391 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.包装信息 数量 100 µg
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