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检测方法 Fluorescence 描述 概述 A convenient and useful assay for the quantitative measurement of cell invasion. Contains 12 cell culture inserts with an 8 µm polycarbonate membrane. This membrane is coated with a thin layer of a biological matrix. This layer prevents noninvasive cells from going through the membrane. Cells are quantified utilizing a highly sensitive fluorescent dye (Excitation: ~485 nm; Emission: ~520 nm). 产品目录编号 QIA129 品牌系列 Calbiochem®
参考 参考 Montell, D.J. 2003. Nat. Rev. Mol. Cell Biol. 4, 13.
Egblad, M., et al. 2002. Nat. Rev. Cancer 2, 161.
Hood, J.D., et al. 2002. Nat. Rev. Cancer 2, 91.
Chatterjee, N., et al. 2001. Environ. Pathol. Toxicol. Oncol. 20, 211.
Koul, D., et al. 2001. Oncogene 20, 6669.
Kleinman, H.K., et al. In Molecular and Cellular Aspects of Basement Membranes, Rohrbach, D.H. and Timpl, R., eds. Academic Press, 1993, pp. 309.
Repesh, L.A. 1989. Invasion Metastasis 9, 192.
Albini, A., et al. 1987. Cancer Res. 47, 3229.
Terranova, V.P., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 465.
Liotta, L.A., 1984. Am. J. Pathol. 117, 339.产品信息 检测方法 Fluorescence 形式 12 Tests 格式 24-well plate 试剂盒包含 24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol. 生物信息 化验时间 24-48 h 样品类型 Culture cells 产品使用声明 预定用途 The Calbiochem® InnoCyte™ Cell Invasion Assay is a quantitative assay for the study of tumor cell invasion in vitro. The assay is useful for discriminating between invasive and non-invasive tumor cell lines and for screening anti-cancer drugs that affect cancer cell invasion. Cell invasion is quantified by labeling the invaded cells with calcein-AM. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C. 无需冷冻 No
补充信息 试剂盒包含 24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol.
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