merck millipore,默克密理博,HTS033M,ChemiSCREEN™ GPR14 Membrane Preparation

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay.	Human	Chem-2	UT / UT1 / GPR14

描述
产品目录编号	HTS033M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ GPR14 Membrane Preparation
概述	TRANSFECTION: Full-length human GPR14 cDNA
Alternate Names	Urotensin II
背景信息	Urotensin II, a cyclic 11-13 residue peptide expressed in motoneurons of the spinal cord, acts as a potent vasoconstrictor (Coulouarn et al., 1998; Ames et al., 1999). The effects of urotensin II are mediated by binding to a GPCR, GPR14, which is expressed in endothelium, smooth muscle, heart and pancreas (Ames et al., 1999). Genetic deletion of GPR14 in mice renders aortae refractile to the contractile activity of urotensin II without changing baseline hemodynamics (Behm et al., 2003). Pharmacological inhibition of urotensin II/GPR14 interactions prevents renal insufficiency following renal artery ligation (Clozel et al., 2004). Thus, GPR14 is an attractive target for a number of cardiovascular diseases. Chemicon's GPR14 Membrane Preparations are ideal tools for screening for antagonists of urotensin II/GPR14 interactions. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-urotensin II. With 10 mg/well GPR14 Membrane Prep and 0.3 nM [125I]-urotensin II, a greater than 20-fold signal-to-background ratio is obtained.

产品信息
格式	Membranes
演示	Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial typically contains 2000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of 125I-labeled urotensin II (0.3 nM input concentration).

应用
应用	Human UT / UT1 / GPR14 GPCR membrane preparation for Radioligand binding Assays.
应用说明	Radioligand binding assay.

生物信息
品种	Human
品种反应性	Human
Entrez基因编号	NM_018949.1
基因符号	UTR UTS2R GPR14 UR-II-R UTR2
GPCR类别	A
宿主细胞	Chem-2
蛋白靶	UT / UT1 / GPR14
靶子系列	Urotensin II
UniProt编号	Q9UKP6
UniProt汇总	FUNCTION: SwissProt: Q9UKP6 # High affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activate a phosphatidylinositol-calcium second messenger system. SIZE: 389 amino acids; 42130 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Most abundant expression in the heart and pancreas. SIMILARITY: SwissProt: Q9UKP6 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with GPR14 membrane preparation.

	10 µg/well
Signal:Background	28.5
Specific Binding (cpm)	58253

1 unit = 10 µg
Bmax for [¹²⁵I] urotensin II binding: 2.7 pmol/mg protein
Kd for [¹²⁵I] urotensin II binding: ~ 0.37 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.