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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB5054 Replaces AB149 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Calretinin Antibody 背景信息 Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc. 产品信息 格式 Serum 控制 - Rat brain cortex, Mesothelioma tissue, Human cerebellum.
演示 Rabbit polyclonal serum contains no preservative. 应用 应用 This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blot:
1:1,000-1:2,000 of a previous lot was used.
Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant rat calretinin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots. 品种反应性 RatHuman Species Reactivity Note Human and rat. Reactivity with other species has not been determined 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001740.3
Entrez基因汇总 Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium. 基因符号 - CAL2
- CALB2
- CAB29
- CR
纯化方法 Unpurified UniProt编号 - P22676
UniProt汇总 FUNCTION: SwissProt: P22676 # Calretinin is a calcium-binding protein which is abundant in auditory neurons.
SIZE: 271 amino acids; 31540 Da
TISSUE SPECIFICITY: Brain.
SIMILARITY: SwissProt: P22676 ## Belongs to the calbindin family. & Contains 6 EF-hand domains.产品使用声明 质量保证 Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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