merck millipore,默克密理博,PS1013,PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb

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Merck Millipore 默克密理愽

宿主
Rb

描述
概述	Recognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr^1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
产品目录编号	PS1013
品牌系列	Calbiochem®
同义词	Anti-KDR/Flk-1, Phospho-Specific (Tyr^1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr^1054/1059)
应用数据	Detection of mouse VEGFR2, phosphorylated at Tyr^1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr^1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.

参考
参考	Meyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081. Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714. Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201. Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.

产品信息
形式	Liquid
配方	In Dulbecco's PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
正控制	Porcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
防腐剂	≤0.1% sodium azide

应用
主要应用	Immunoblotting (Western Blotting)

生物信息
免疫原	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1054/1059 phosphorylation sites of mouse and human VEGFR-2
宿主	Rabbit
同种型	IgG
品种	mouse

储存和货运信息
货运代码	Blue Ice Only
毒性	Irritant
储存	-20°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following initial thaw, aliquot and freeze (-20°C).

merck millipore,默克密理博,PS1013,PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb

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