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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9856 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-TorsinB Antibody 产品信息 格式 Serum 控制 - Western blot: brain and liver. Immunocytochemistry: SH-SY5Y cells.
演示 Liquid. 应用 应用 Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment.
Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA.
Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide from torsinB. 宿主 Rabbit 特异性 TorsinB. The antibody reacts with the ~38 kDa protein. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_014506.1
基因符号 - DQ1
- TOR1B
- MGC4386
纯化方法 Serum UniProt编号 - O14657
UniProt汇总 FUNCTION: SwissProt: O14657 # May serve as a molecular chaperone assisting in the proper folding of secreted and/or membrane proteins (By similarity).
SIZE: 336 amino acids; 37979 Da
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen (By similarity).
TISSUE SPECIFICITY: Widely expressed with low levels in brain. Not detected in fetal brain.
SIMILARITY: SwissProt: O14657 ## Belongs to the clpA/clpB family. Torsin subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,513033,PD 168393 - CAS 194423-15-9 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,ABE500,Anti-WBP-7 Antibody
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