merck millipore,默克密理愽
  • merck millipore,默克密理博,PMEF-CFX,EmbryoMax Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3

  • merck millipore,默克密理博,PMEF-CFX,EmbryoMax Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3

    产品名称：EmbryoMax Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3
    产品型号：PMEF-CFX
    The PMEF feeders are mitotically inactivated by treatment with irradiation & supplied at passage 3. These fibroblasts can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels.

    merck millipore,默克密理博,PMEF-CFX,EmbryoMax Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3

  • 产品介绍
  • merck millipore,默克密理博,PMEF-CFX,EmbryoMax Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3

    重要规格表

    主要应用
    Cult
    描述
    产品目录编号 PMEF-CFX
    商名
    • Specialty Media
    • Embryomax
    描述 EmbryoMax® Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3
    概述 These PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

    • 6 x 60mm dishes
    • 2 x T75 flasks
    • 2 x 6 well plates

    Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.
    Alternate Names
    • Non drug resistant cells from CF-1 mice- Irradiated
    背景信息 The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
    应用
    应用 The PMEF feeders are mitotically inactivated by treatment with irradiation & supplied at passage 3. These fibroblasts can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels.
    主要应用
    • Stem Cell Culture
    生物信息
    细胞系类型
    • Primary Mouse Embryo Fibroblasts
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
    包装信息
    数量 5 vials
    包装 5-6x106 ea

    merck millipore,默克密理博,PMEF-CFX,EmbryoMax Primary Mouse Embryo Fibroblasts, Strain CF1, Irradiated, passage 3

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