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merck millipore,默克密理博,MCH200110,Muse™RFP-LC3 Reporter Autophagy Assay Kit
产品名称：Muse™RFP-LC3 Reporter Autophagy Assay Kit
产品型号：MCH200110
The Muse RFP-LC3 Reporter Autophagy Assay kit includes one immortalized RFP-LC3 reporter cell line to measure and track the levels of LC3 within the cell. The RFP-LC3 reporter cell line provided in t More>>
merck millipore,默克密理博,MCH200110,Muse™RFP-LC3 Reporter Autophagy Assay Kit
- 产品介绍
描述 产品目录编号 MCH200110 商名 - Muse
描述 Muse™RFP-LC3 Reporter Autophagy Assay Kit 概述 EMD Millipore’s Muse™ RFP-LC3 Reporter Autophagy Assay kit provides a quantitative solution for the study of autophagy and potency evaluation of autophagy inducers using flow cytometry. This kit has four unique features:
1)The use of selective permeabilization solution discriminates cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins, while protecting LC3 which has been sequestered into the autophagosome from degradation;
2) A monomeric RFP is used as a reporter to facilitate the translocation of the fusion protein. Other forms of RFP are not preferred as they form dimers and aggregate when over-expressed in the cells, preventing their extraction from the cytoplasm and impairing the ability to measure translocation by flow cytometry;
3) Since autophagy is a constitutive cellular degradation process, the use of an autophagy detection reagent (Autophagy Reagent A) will prevent the lysosomal degradation of LC3, allowing its quantification by flow cytometry;
4) The monomeric RFP used in our fusion protein to LC3 is attached on the 5' end (N-terminal fusion), protecting the RFP from Atg4 cleavage, thus, allowing its visualization within the autophagosomes
To assist in autophagy study, U20S (human osteosarcoma) cells are stably transfected, which are suitable for quantitative and higher throughput analysis of autophagy. Data generated using the Muse™ Cell Analyzer along with the corresponding Muse™ software module provides statistical values measuring:
• Mean Autophagy Value (for both control and test samples)
• Autophagy Induction Ratio (Test sample fluorescence relative to control)
• Percentage of cells with increased autophagy (Test sample versus control)
EMD Millipore’s Muse™ RFP-LC3 Reporter Autophagy Assay kit includes one immortalized RFP-LC3 reporter cell line to measure and track the levels of LC3 within the cell. The RFP-LC3 reporter cell line provided in the kit has been carefully optimized along with the corresponding reagents to ensure optimal performance. This kit contains reagents A and B, along with an assay buffer to provide researchers a complete solution for autophagy analysis. The autophagy detection reagents and cell line have been optimized together to ensure the ability to measure and discriminate between cytosolic and lipidated LC3 to accurately measure the autophagic process. Sufficient enabling reagents are provided to perform 100 tests
The Muse™ RFP-LC3 Reporter Autophagy Assay kit is optimized on the Muse™ Cell Analyzer. The Muse™ System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse™ Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.
For Research Use Only; not for use in diagnostic procedures.应用 应用 The Muse® RFP-LC3 Reporter Autophagy Assay kit includes one immortalized RFP-LC3 reporter cell line to measure and track the levels of LC3 within the cell. The RFP-LC3 reporter cell line provided in the kit has been optimized along with the corresponding reagents to ensure optimal performance. 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 100 Tests
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