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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABS275 描述 Anti-Gli3 Antibody, clone 5E1 Alternate Names - GLI3-83
- Transcriptional activator GLI3
- GLI3 form of 190 kDa
- GLI3-190
- GLI3FL
- GLI3 C-terminally truncated form
- GLI3 form of 83 kDa
背景信息 Gli3 can function as both a transcriptional activator and repressor of the sonic hedgehog (Shh) pathway. The full-length Gli3 form (GLI3FL) becomes an activator (GLI3A) after phosphorylation and nuclear translocation. The C-terminally truncated form (GLI3R), acts as a repressor. Proper balance between the Gli3 activator and the repressor specifies limb digit number and identity during development. In concert with TRPS1, Gli3 plays a role in activating chondrocyte proliferation. Gli3 is expressed in several normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Gli3 defects are associated with Greig cephalo-poly-syndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), polydactyly postaxial type A1 (PAPA1), polydactyly postaxial type B polydactyly (PAPB), and polydactyly preaxial type 4 (POP4). 产品信息 格式 Purified 控制 - Human thymus tissue lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 This Gli3 antibody is validated for use in WB, ICC & IHC for the detection of the Gli3 protein. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis: A representative lot detected Gli3 in PK-15, COS-1, and TM4 cells cotransfected with recombinant Gli3 (Hunt, R., et al. (2007). Hybridoma. 27(4):231-240.).
Immunohistochemistry Analysis: A representative lot detected Gli3 in mouse embryo cross-sections (Laht, S., et al. (2008). Hybridoma. 27(3):167-174.).生物信息 免疫原品种 Recombinant protein corresponding to the putative repressive motif of human GLI3. 克隆 5E1 宿主 Mouse 特异性 This antibody recognizes the truncated, repressor form and the full length protein of human Gli3. 同种型 IgMκ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000159.3
基因符号 - GLI3
纯化方法 Purified by ion-exchange chromatography UniProt编号 - P10071
产品使用声明 质量保证 Evaluated by Western Blotting in human thymus tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected in Gli3 in 10 µg of human thymus tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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