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纯度 ≥95% by SDS-PAGE 描述 概述 Recombinant, human VEGF165 (a 165 amino acid splice varian of VEGF) expressed under serum-free conditions in Spodoptera frugiperda cells and purified via a proprietary sequential chromatrography methods. VEGF165 binds to the connective tissue growth factor (CTGF) and the resulting complex has no angiogenic activity. Degradation of CTGF by matrix metalloproteinases results in VEGF-induced angiogenesis during embryonic development and pathological disease states. The recombinant disulfide-linked homodimeric human VEGF is glycosylated and, thus, migrates as a ~42 kDa protein in SDS-PAGE under non-reducing conditions. 产品目录编号 PF074 品牌系列 Calbiochem®
同义词 Vascular Permeability Factor 参考 参考 Hashimoto, G., et al. 2002. J. Biol. Chem. In Press.
Soker, S., et al. 1998. Cell 92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
Brown, L., et al. 1992. Kidney International 42, 1457.
Dvorak, H., et al. 1991. J. Exp. Med. 174, 1275.
Conn, G., et al. 1990. PNAS 87, 1323.
Leung, D.W., et al. 1989. Science 246, 1306.产品信息 形式 Lyophilized 配方 Carrier free. Molar Mass 38600 防腐剂 None 应用 数据设置错误。 未找到<collection_feature_application_id-PROLIF>。
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生物信息 生物活性 EC₅₀ of 1-6 ng/ml as determined by the dose dependent proliferation of human ubilical vein epithelial cells (HUVEC) 纯度 ≥95% by SDS-PAGE 理化信息 污染物 Endotoxins: ≤100 pg/µg VEGF 溶解度 PBS with 0.1% HSA or BSA 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
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