- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
描述 概述 Recombinant, human pro-MMP-3 purified from cell culture supernatant. May be used as a positive control or standard for zymographic analysis, or substrate assay. Requires activation for immunoblotting, prior to use. M.W. 57000/58000. 产品目录编号 PF063 品牌系列 Calbiochem®
参考 参考 Sole, S., et al. 2004. J. Neuropathol. Exp. Neurol. 63, 338.
Galazka, G., et al. 1996. Biochem. 35, 11221.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Netzel-Arnett, S., et al. 1991. Anal. Biochem. 195, 86.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Zymography References
Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259.
Kleiner, D.E. and Stetler-Stevenson W.G. 1994. Anal. Biochem. 218, 325.
Heussen, C. and Dowdle, E.B. 1980. Anal. Biochem. 102, 196.
Substrate Cleavage Assay References
Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259.
Netzel-Arnett, S., et al. 1991. Anal. Biochem. 195, 86.产品信息 活性 The activity of proenzyme MMP 3 was measured by substrate cleavage assay using 0.5 mM thiopeptiolide (Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-Oet) as a substrate. The activity was also assessed by degradation of a peptide substrate (DNP-PYAYWMR) using activated MMP-3 as measured by HPLC. EC号 3.4.24.17 形式 Lyophilized 配方 Lyophilized from 100 mM NaCl, 50 mM HEPES, pH 7.3. Molar Mass 57000 应用 应用参考 ZYMOGRAPHY REFERENCES Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259. Kleiner, D.E. and Stetler-Stevenson W.G. 1994. Anal. Biochem. 218, 325. Heussen, C. and Dowdle, E.B. 1980. Anal. Biochem. 102, 196. SUBSTRATE CLEAVAGE ASSAY REFERENCE Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259. Netzel-Arnett S, et al. 1991. Anal. Biochem. 195, 86. 主要应用 Immunoblotting (Western Blotting)
未找到<collection_feature_application_id-SCA>。
未找到<feature_application_id_2-SCA>。
Zymography
生物信息 纯度 ≥95% by SDS-PAGE 理化信息 溶解度 H₂O 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following reconstitution, aliquot into siliconized vials and freeze (-70°C).
上一件merck millipore产品：merck millipore,默克密理博,AG980,SOX2 Control Peptide
下一件merck millipore产品：merck millipore,默克密理博,371729,Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb
相关产品
merck millipore,默克密理博,565780,β-Secretase Inhibitor III - Calbiochem
merck millipore,默克密理博,ABC509,Anti-DEK Antibody
merck millipore,默克密理博,AB16982,Anti-Fas Ligand Antibody
merck millipore,默克密理博,MABS65,Anti-Insulin Receptor Antibody, beta subunit, clone CT-3
merck millipore,默克密理博,AG980,SOX2 Control Peptide
merck millipore,默克密理博,371729,Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb
merck millipore,默克密理博,ST1579,Anti-FADS1 Mouse mAb (2D9)
merck millipore,默克密理博,ABN209,Anti-TTC8 Antibody
