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merck millipore,默克密理博,06-1299,Anti-Pontin (Tip49) Antibody
产品名称：Anti-Pontin (Tip49) Antibody
产品型号：06-1299
Use Anti-Pontin (Tip49) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Pontin (Tip49) also known as 49 kDa TATA box-binding protein-interacting protein or Nuclear matrix protein More>>
merck millipore,默克密理博,06-1299,Anti-Pontin (Tip49) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1299 描述 Anti-Pontin (Tip49) Antibody Alternate Names - TIP60-associated protein 54-alpha
- 49 kDa TATA box-binding protein-interacting protein
- 49 kDa TBP-interacting protein
- 54 kDa erythrocyte cytosolic protein
- INO80 complex subunit H
- NMP 238
- Nuclear matrix protein 238
- Pontin 52
- RuvB (E coli homolog)-like 1
- RuvB-like 1
- RuvB-like 1 (E. coli)
- TATA binding protein interacting protein 49 kDa
背景信息 Pontin/TIP49 (also known as Pontin 52, TIP49a, or 49 kDa TATA box-binding protein-interacting protein) is a member of the ruvB family. Members of this family are known to have ATP-dependent DNA helicase and a single-stranded, DNA-stimulated ATPase activities. Pontin participates in select transcriptional activation as a component of the NuA4 histone acetyltransferase complex that is thought to be necessary for the activation of specific transcription pathways related to oncogenesis. Pontin is a critical factor in cell proliferation and, when present on the cell surface, may bind to plasminogen and upregulate the activation of plasminogen. Expression of pontin is considered ubiquitous; however significantly high levels have been reported in skeletal muscle, testis, and heart tissue. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-Pontin (Tip49) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Pontin (Tip49) also known as 49 kDa TATA box-binding protein-interacting protein or Nuclear matrix protein 238. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pontin (TIP49) in NIH/3T3, HeLa, and A431 cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human pontin. 表位 Recognizes Pontin/Tip49 at a region near the amino terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Pontin/Tip49 at a region near the amino terminus 品种反应性 CanineHumanMouse Species Reactivity Note Demonstrated to react with human and mouse.
Predicted to react with canine based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003698
基因符号 - TIP49a
- ECP-54
- ECP54
- INO80H
- NMP238
- PONTIN
- Pontin52
- RVB1
- Rvb1
- TAP54-alpha
- TIH1
- TIP49
- TIP49A
纯化方法 Affinity Purified UniProt编号 - Q9Y265
UniProt汇总 FUNCTION: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
May be able to bind plasminogen at cell surface and enhance plasminogen activation.
Essential for cell proliferation.
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
SUBUNIT STRUCTURE: Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. Interacts with OFD1.
SUBCELULAR LOCATION: Nucleus matrix. Nucleus › nucleoplasm. Cytoplasm. Membrane. Cytoplasm › cytoskeleton › centrosome. Note: Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
TISSUE SPECIFICTY: Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
DOMAIN: Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
MISCELLANEOUS: High level of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.
SEQUENCE SIMILARITIES: Belongs to the ruvB family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Pontin (Tip49) in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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