merck millipore,默克密理博,PF023,MMP-2, Active, Human, Recombinant, CHO Cells

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merck millipore,默克密理博,PF023,MMP-2, Active, Human, Recombinant, CHO Cells
产品名称：MMP-2, Active, Human, Recombinant, CHO Cells
产品型号：PF023

merck millipore,默克密理博,PF023,MMP-2, Active, Human, Recombinant, CHO Cells

产品介绍

merck millipore,默克密理博,PF023,MMP-2, Active, Human, Recombinant, CHO Cells

描述
概述	Full-length, recombinant, human pro-MMP-2 expressed in CHO cells that is subsequently activated by APMA. APMA is removed through a desalting column. The substrate specificity for MMP-2 includes collagen (types IV, V, VII, and X), elastin, and gelatin (type I). The presence of TIMP-2 inhibitor prevents degradation of the MMP-2 C-terminal regulatory domain. TIMP-2 is also an inhibitor of proteolysis and will inhibit the activity of the enzyme. Useful for immunoblotting, substrate cleavage assay and zymography.
产品目录编号	PF023
品牌系列	Calbiochem®
同义词	Gelatinase A, Active Matrix Metalloproteinase 2

参考
参考	Liepnisch, E., et al. 2003. J. Biol. Chem. 278, 25982. Parsons, S.L., et al. 1997. Br. J. Surg. 84, 160. Backstrom, J.R., et al. 1996. J. Neuro. 16, 7910. Lim, G.P., et al. 1996. J. Neurochem. 67, 251. Xia, T., et al. 1996. Biochim. Biophys. Acta 1293, 259. Chandler, S., et al. 1995 Neuroscience Letters 201, 226. Sang, Q.X., et al. 1995. Biochim. Biophys. Acta 1251, 99. Kenagy, R.D. and Clowes, A.W. 1994. In Inhibition of Matrix Metalloproteinases: Therapeutic Potential. Greenwald, R.A. and Golub L.M., Eds, 465. Zempo, N., et al. 1994. J. Vasc. Surg. 20, 217. Birkedal-Hansen, H. 1993. J. Periodontol. 64, 484. Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434. Delaisse, J-M. and Vaes, G. 1992. In Biology and Physiology of the Osteoclast. B.R. Rifkin and C.V. Gay, Eds., 290. Jeffrey, J.J. 1992. In Wound Healing: Biochemical and Clinical Aspects. R.F. Diegelmann and W.J. Lindblad, Eds., 194. Jeffrey, J.J. 1991. Semin. Perinatol. 15, 118. Liotta, L.A., et al. 1991. Cell 64, 327. Harris, E. 1990. N. Engl. J. Med. 322, 1277.

参考

Liepnisch, E., et al. 2003. J. Biol. Chem. 278, 25982.
Parsons, S.L., et al. 1997. Br. J. Surg. 84, 160.
Backstrom, J.R., et al. 1996. J. Neuro. 16, 7910.
Lim, G.P., et al. 1996. J. Neurochem. 67, 251.
Xia, T., et al. 1996. Biochim. Biophys. Acta 1293, 259.
Chandler, S., et al. 1995 Neuroscience Letters 201, 226.
Sang, Q.X., et al. 1995. Biochim. Biophys. Acta 1251, 99.
Kenagy, R.D. and Clowes, A.W. 1994. In Inhibition of Matrix Metalloproteinases: Therapeutic Potential. Greenwald, R.A. and Golub L.M., Eds, 465.
Zempo, N., et al. 1994. J. Vasc. Surg. 20, 217.
Birkedal-Hansen, H. 1993. J. Periodontol. 64, 484.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Delaisse, J-M. and Vaes, G. 1992. In Biology and Physiology of the Osteoclast. B.R. Rifkin and C.V. Gay, Eds., 290.
Jeffrey, J.J. 1992. In Wound Healing: Biochemical and Clinical Aspects. R.F. Diegelmann and W.J. Lindblad, Eds., 194.
Jeffrey, J.J. 1991. Semin. Perinatol. 15, 118.
Liotta, L.A., et al. 1991. Cell 64, 327.
Harris, E. 1990. N. Engl. J. Med. 322, 1277.

产品信息
活性	ΔΑ₄₀₅/hour/µg protein: ≥ 7.0 in standard thiopeptolide hydrolysis assays.
EC号	3.4.24.24
形式	Liquid
配方	In 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% BRIJ^®-35 Detergent, pH 7.5.
Molar Mass	66000
防腐剂	None

生物信息
纯度	≥90% by SDS-PAGE; contains 9% TIMP-2
浓缩标签	Please refer to vial label for lot-specific concentration

储存和货运信息
货运代码	Dry Ice Only
毒性	Standard Handling
储存	≤ -70°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following initial use, aliquot into siliconized vials and freeze (-70°C).