merck millipore,默克密理博,MABT208,Anti-Fyn Antibody, clone EPR5500, rabbit monoclonal

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merck millipore,默克密理博,MABT208,Anti-Fyn Antibody, clone EPR5500, rabbit monoclonal
产品名称：Anti-Fyn Antibody, clone EPR5500, rabbit monoclonal
产品型号：MABT208
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Fyn Antibody, clone EPR5500, Rabbit Monocl More>>

merck millipore,默克密理博,MABT208,Anti-Fyn Antibody, clone EPR5500, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABT208,Anti-Fyn Antibody, clone EPR5500, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABT208
Replaces	04-353
描述	Anti-Fyn Antibody, clone EPR5500, rabbit monoclonal
Alternate Names	tyrosine-protein kinase Fyn FYN oncogene related to SRC, FGR, YES Proto-oncogene c-Fyn Proto-oncogene Syn Src-like kinase p59-Fyn c-syn protooncogene OKT3-induced calcium influx regulator protein-tyrosine kinase fyn proto-oncogene tyrosine-protein kinase fyn src/yes-related novel tyrosine kinase p59fyn(T)
背景信息	Fyn is a member of the protein-tyrosine kinase oncogene family. It is a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein.

产品信息
格式	Unpurified
控制	Ramos, Jurkat, and NIH/3T3 cell lysates
演示	Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Fyn Antibody, clone EPR5500, Rabbit Monoclonal is validated for use in Western Blotting for the detection of Fyn.
主要应用	Western Blotting

生物信息
免疫原品种	Synthetic peptide corresponding to human Fyn.
克隆	EPR5500
宿主	Rabbit
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with Human, Rat, and Mouse. Species reactivity is based on WB analysis.
抗体类型	Monoclonal Antibody
Entrez基因编号	P06241
基因符号	EC 2.7.10 SLK MGC45350 SYN EC 2.7.10.2 OTTHUMP00000017914 OTTHUMP00000017915 OTTHUMP00000017917 OTTHUMP00000226354 OTTHUMP00000226453
纯化方法	Unpurified
UniProt编号	P06241
UniProt汇总	FUNCTION: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. COFACTOR: Manganese. ENZYME REGULATION: Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site. SUBUNIT STRUCTURE: Interacts (via its SH3 domain) with PIK3R1 and PRMT8. Interacts with FYB, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated) By similarity. Interacts with TOM1L1 (phosphorylated form). Interacts with KDR (tyrosine phosphorylated) By similarity. Interacts with SH2D1A and SLAMF1. Interacts (via its SH3 domain) with HEV ORF3 protein. Interacts with ITCH; the interaction phosphorylates ITCH and negatively regulates its activity. Interacts with FASLG. Interacts with RUNX3. Interacts with KIT. Interacts with EPHA8; possible downstream effector of EPHA8 in regulation of cell adhesion. Interacts with PTK2/FAK1; this interaction leads to PTK2/FAK1 phosphorylation and activation. Interacts with CAV1; this interaction couples integrins to the Ras-ERK pathway. SUBCELLULAR LOCATION: Cell membrane. Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking. Ref.23 TISSUE SPECIFICITY: Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. PTM: Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state By similarity. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Palmitoylation at Cys-3 and Cys-6 regulates subcellular location By similarity. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

产品使用声明
质量保证	Evaluated by Western Blot in Ramos, Jurkat, and NIH/3T3 cell lysates. Western Blot Analysis: A 1:1,000-10,000 dilution of this antibody was used to detect Fyn in Ramos, Jurkat, and NIH/3T3 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.