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检测方法 Fluorogenic 描述 概述 A sensitive fluorogenic assay (Exc. max: ~325 nm; Emi. max.: ~393 nm) for the measurement of gelatinases (MMP-2 and MMP-9). Also useful for the screening of gelatinase inhibitors. 产品目录编号 CBA003 品牌系列 Calbiochem®
同义词 MMP-2/MMP-9 Activity Assay Kit, Fluorogenic 应用数据 
Human Pro-MMP-2 (supplied) was activated as outlined in the Reagent Preparation section above and assayed for activity as outlined in the Detailed Protocol, incubating for a total of 3 h.
Activated MMP-9 (Cat. No. PF140) was assayed without APMA at 37°C for 3 h according to the Detailed Protocol outlined above.
Serum from 5 different donors was diluted 1:5 in Activation Buffer and HT1080 culture supernatant was diluted 1:3 with Activation Buffer. All samples were incubated at 37°C for 3 h according to the Detailed Protocol outlined above.参考 参考 Lauer-Fields, J.L., et al. 2003. J. Biol. Chem. 278, 18140.
Chang, C., and Werb, Z. 2001. Trends Cell Biol. 11, S37.
Kleiner, D.E., and Stetler-Stevenson, W.G. 1999. Cancer Chemother. Pharmacol. 43 (suppl.), S42.
Cockett, M.I., et al. 1998. Biochem. Soc. Symp. 63, 295.
Olson, M.W., et al. 1997. J. Biol. Chem. 272, 29975.
Will, H., et al. 1996. J. Biol. Chem. 271, 17119.
MacDougall, J.R., et al. 1995. Cancer Metastasis Rev. 14, 351.
Liotta, L.A., et al. 1990. Semin. Cancer Biol. 1, 99.产品信息 检测方法 Fluorogenic 形式 96 Tests 格式 96-well plate 试剂盒包含 Gelatinase Substrate, pro-MMP-2 Positive Control, Inhibitor, APMA, Assay Buffer, 96-Well Plate, Plate Sealer, and a user protocol 正控制 MMP-9 (Cat. No. PF140) 生物信息 化验时间 2 - 6 h 样品类型 Cell lysate, culture supernatant, tissue extract, plasma, serum, purified enzyme 理化信息 灵敏度 20 ng/ml 安全信息 R相 R: 28-33-36/38-40-43-50/53-60
Very toxic if swallowed.
Danger of cumulative effects.
Irritating to eyes and skin.
Limited evidence of a carcinogenic effect.
May cause sensitization by skin contact.
Very toxic to aquatic organisms; may cause long-term adverse effects in the aquatic environment.
May impair fertility.S相 S: 22-26-36/37-57-60-61
Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
Use appropriate containment to avoid environmental contamination.
This material and its container must be disposed of as hazardous waste.
Avoid release to the environment. Refer to special instructions/safety data sheet.产品使用声明 预定用途 The Calbiochem® InnoZyme™ Gelatinase (MMP-2/MMP-9) Activity Assay Kit is designed for measuring gelatinase (MMP-2 and MMP-9) activity in a variety of samples. It is most suitable for screening gelatinase inhibitors using purified enzymes. It is not as sensitive as zymography, but it is still sufficient for measuring activity in biological samples (e.g., serum and plasma), culture supernatant, or tissue extracts. The assay utilizes a triple-helical peptide substrate that is highly selective for MMP-2 and MMP-9. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon arrival store the MMP-2 at -70°C and all other contents of the kit at -20°C. 避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Gelatinase Substrate, pro-MMP-2 Positive Control, Inhibitor, APMA, Assay Buffer, 96-Well Plate, Plate Sealer, and a user protocol
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