merck millipore,默克密理博,MABT143,Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, rabbit monoclonal

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merck millipore,默克密理博,MABT143,Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, rabbit monoclonal
产品名称：Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, rabbit monoclonal
产品型号：MABT143
Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, Rabbit is an antibody against Alpha-Actinin 3 for use in WB, IC, IH(P), FC.

merck millipore,默克密理博,MABT143,Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABT143,Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB, ICC	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABT143
描述	Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, rabbit monoclonal
Alternate Names	Alpha-actinin skeletal muscle isoform 3 actinin, alpha 3 F-actin cross-linking protein alpha-actinin-3 alpha-actinin skeletal muscle
背景信息	The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibers. Absence of alpha-actinin-3 is not associated with an obvious disease phenotype, raising the possibility that ACTN3 is functionally redundant in humans, and that alpha-actinin-2 is able to compensate for alpha-actinin-3 deficiency (1). Alpha-Actinin-3 is specifically expressed in fast-twitch myofibers responsible for generating force at high velocity. The absence of a disease phenotype secondary to alpha-actinin-3 deficiency is likely due to compensation by the homologous protein, alpha-actinin-2. However, the high degree of evolutionary conservation of ACTN3 suggests function(s) independent of ACTN2. Highly significant associations between ACTN3 genotype and athletic performance have been demonstrated in both male and female athletes.

产品信息
格式	Diluted Culture Supernatant
控制	Human skeletal muscle tissue lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-Alpha-Actinin 3 Antibody, clone EP2531Y, Rabbit is an antibody against Alpha-Actinin 3 for use in WB, IC, IH(P), FC.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: 1:100-250 dilution from a representative lot was used in IC. Flow Cytometry Analysis: 1:200 dilution from a representative lot was used in FC. Immunohistochemistry Analysis: 1:100-250 dilution from a representative lot detected Alpha-Actinin 3 in human muscle tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to human Alpha-Actinin 3 at the N-terminus.
表位	N-terminus
克隆	EP2531Y
宿主	Rabbit
特异性	This antibody recognizes Alpha-Actinin 3 at the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
基因符号	MGC117005 OTTHUMP00000219054 MGC117002 OTTHUMP0000021904
纯化方法	Unpurified
UniProt编号	Q08043
UniProt汇总	FUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. SUBUNIT STRUCTURE: Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1. TISSUE SPECIFICITY: Expressed only in a subset of type 2 skeletal muscle fibers. POLYMORPHISM: About 18% of the world population lack a functional ACTN3 due to a stop codon polymorphism at position 577. The absence of a functional ACTN3 expression is not correlated with a disease state. SEQUENCE SIMILARITIES: Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats. SEQUENCE CAUTION: The sequence AP002748 differs from that shown. Reason: According to the human genome assembly there is a stop codon in position 577 which is only found in 18% of the world population.

产品使用声明
质量保证	Evaluated by Western Blot in human skeletal muscle tissue lysate. Western Blot Analysis: 1:10,000 dilution of this antibody detected Alpha-Actinin 3 in human skeletal muscle tissue lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.