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merck millipore,默克密理博,MABS64,Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2
产品名称：Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2
产品型号：MABS64
Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.
merck millipore,默克密理博,MABS64,Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABS64 描述 Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 Alternate Names - Csaids binding protein
- mitogen-activated protein kinase 14
- cytokine suppressive anti-inflammatory drug binding protein
- stress-activated protein kinase 2A
- Cytokine suppressive anti-inflammatory drug-binding protein
- CSAID-binding protein
- MAP kinase MXI2
- MAX-interacting protein 2
- Mitogen-activated protein kinase p38 alpha
- p38 MAP kinase
- p38 mitogen activated protein kinase
- MAP kinase p38 alpha
背景信息 The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation. 产品信息 格式 Purified 控制 - Anisomycin treated and untreated HeLa cell lysate
演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells. 生物信息 免疫原品种 Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182. 表位 Phosphorylated Thr180 and Tyr182 克隆 6E5.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182. 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human and mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001306.1
基因符号 - EXIP
- SAPK2a
- MAPK14
- CSBP1
- CSBP2
- CSPB1
- PRKM14
- PRKM15
- p38ALPHA
- RK
- Mxi2
修改 - Phosphorylation
纯化方法 Protein G purfied UniProt编号 - Q16539
UniProt汇总 FUNCTION: Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
COFACTOR: Magnesium.
SIZE: 360 amino acids; 41293 Da
SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (by similarity). Interacts with NP60.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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