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品种反应性 主要应用 宿主 格式 抗体类型 H, M IHC, WB R Purified Monoclonal Antibody 描述 产品目录编号 MABS465 描述 Anti-Nampt, clone 14A.5 Antibody Alternate Names - Visfatin
- PBEF
- Nicotinamide phosphoribosyltransferase
- NAmPRTase
- Nampt
- Pre-B-cell colony-enhancing factor 1 homolog
背景信息 Nicotinamide phosphoribosyltransferase (NAMPT) is a member of the NAPRTase family. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. NAMPT is localized in the cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. 产品信息 格式 Purified 演示 Purified rat monoclonal IgG2aκ in buffer containing PBS without preservatives. 应用 应用 Anti-Nampt antibody, clone 14A.5 is an antibody against Nampt for use in western blotting, IHC, inhibition & ELISA. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Nampt in Anti-CD3epsilon stimulated mouse spleen cell lysate (Prof. Oberdan Leo, Universite Libre de Bruxelles.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Nampt in mouse spleen tissue lysate (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).
Inhibition Analysis: A representative lot from an independent laboratory was inhibited by a synthetic peptide corresponding to murine Nampt in total liver extracts (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Nampt in CD11c positive mouse spleen sections (Prof. Oberdan Leo, Universite Libre de Bruxelles.).
ELISA Analysis: A representative lot from an independent laboratory detected recombinant Nampt peptides (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).生物信息 免疫原品种 Recombinant protein corresponding to mouse Nampt. 克隆 14A5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 同种型 IgG2aκ 品种反应性 HumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_067499
基因符号 - Pbef1
- Nampt
纯化方法 Protein G purified UniProt编号 - Q99KQ4
产品使用声明 质量保证 Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nampt in 10 µg of Raji cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
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