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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABS148 描述 Anti-PKLR Antibody, clone 11F12.1 Alternate Names - Red cell/liver pyruvate kinase
- Pyruvate kinase isozymes R/L
- Pyruvate kinase 1
- R-type/L-type pyruvate kinase
背景信息 PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). 产品信息 格式 Purified 控制 - HepG2 cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect PKLR using this Anti-PKLR Antibody, clone 11F12.1 validated for use in Western Blotting & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blot Analysis: A 1:1,000 dilution from a representative lot detecte PKLR in 10 µg of rat liver tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PKLR in rat liver hepatocytes.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human PKLR. 克隆 11F12.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2bκ 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human, Rat, and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000289
基因符号 - PKL
- PKLR
- PK1
纯化方法 Protein G purified UniProt编号 - P30613
产品使用声明 质量保证 Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected PKLR in 10 µg of HepG2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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